Diamond

Acft.V SimtMaqpi 0«t WD I-1 60 |im fdfttV 50 RM» GSE tl 1 »micronBDD

Figure 8.24. SEM images of (a,b) selective diamond onto a (c) tungsten microelectrode (quartz insulator); final tip diameter, 35 ^m. Source: From Ref. 28.

Park and coworkers studied the neurotransmitter,31 endogenous norepinephrine (NE) release with the evoked contractile response of a mesenteric artery from a healthy Sprague Dawley rat using continuous amperometry with a diamond microelectrode and video microscopy. Figure 8.26 A-D shows the diagram of the diamond microelectrode insulated with polypropylene. Figure 8.26 F-G show the video micrographs of a mesenteric artery (F) before and (G) after a 20-Hz electrical stimulation. The experiments demonstrated recording of NE released from sympathetic nerves at the surface of a mesenteric artery and the evoked response using the instrument shown in Fig. 8.26.

Figure 8.25. (A) Schematic of the in vitro, extracellular recording/stimulation apparatus. The diamond electrode is pressed into the insulation sheath over a neural cell of interest to specifically record or stimulate that cell. (B) Experimental setup of in vitro detection of serotonin at the I2 muscle of the Aplysia — the cerebral ganglion is desheathed in a Petri dish with inlet and outlet flow of high divalent cationic solution. An intracellular electrode is placed inside the metacerebral cell (MCC) and a diamond electrode is placed within the muscle fibers of the I2 muscle. Source: From Ref. 28.

Figure 8.25. (A) Schematic of the in vitro, extracellular recording/stimulation apparatus. The diamond electrode is pressed into the insulation sheath over a neural cell of interest to specifically record or stimulate that cell. (B) Experimental setup of in vitro detection of serotonin at the I2 muscle of the Aplysia — the cerebral ganglion is desheathed in a Petri dish with inlet and outlet flow of high divalent cationic solution. An intracellular electrode is placed inside the metacerebral cell (MCC) and a diamond electrode is placed within the muscle fibers of the I2 muscle. Source: From Ref. 28.

Figure 8.26. (A) Diagram of the conically shaped diamond microelec-trode insulated with polypropylene. SEM images of the polypropylene-insulated diamond at (B) lower and (C) higher magnification. Top-view SEM images of the diamond film morphology without (D) and with (E) the polypropylene insulation layer. Video micrographs showing a mesenteric artery (F) before and (G) after a 20-Hz electrical stimulation. Source: From Ref. 31.

Figure 8.26. (A) Diagram of the conically shaped diamond microelec-trode insulated with polypropylene. SEM images of the polypropylene-insulated diamond at (B) lower and (C) higher magnification. Top-view SEM images of the diamond film morphology without (D) and with (E) the polypropylene insulation layer. Video micrographs showing a mesenteric artery (F) before and (G) after a 20-Hz electrical stimulation. Source: From Ref. 31.

0 0

Post a comment