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Horse radish peroxidase /

Figure 8.23. Schematic representation of the two-layer structure of the modified NCD surface. Source: From Ref. 30.

Horse radish peroxidase /

Enzymatic layer

Organic layer

Figure 8.23. Schematic representation of the two-layer structure of the modified NCD surface. Source: From Ref. 30.

on the modified NCD for 15 days. This modified NCD biosensor can be potentially used for in vivo applications or for clinical methodologies that are based on the measurement of hydrogen peroxide produced during the analysis.

Halpern and coworkers studied neurodynamics in an animal model, Aplysia calfornica, using diamond microelectrodes.28 The microelectrodes (about 30 ^m diameter) were made using hot-filament-assisted CVD to deposit boron-doped diamond onto a tungsten microelectrode (Fig. 8.24). The microelectrode was used to study the extracellular stimulation of the single neuron, B4 or B5 (Fig. 8.25(A)). And recording activity and stimulating of the cells were observed by the diamond electrode through fast scan cyclic voltammetry (FSCV). Further, the same method was able to quantitatively record increased levels of serotonin at the I2 muscle upon specific stimulation of MCC (Fig. 8.25(B) shows the experimental setup).

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