Different Proteins in Alternation with Polyions

An elaboration of the assembly technique for a variety of proteins makes it possible to construct multicomponent protein films (superlattices) [77, 79, 83]. We have described the formation of two types of superlattices:

1. The alternation of similarly charged proteins at identical pH conditions; both positively charged myoglobin and lysozyme in alternation with polyan-ion {myoglobin+/PSS/lysozyme+/PSS}; negatively charged glucose oxidase (GOx) and glucoamylase (GA) with polycation {glucose oxidase-/PDDA/gluco-amylase-/PDDA}.

2. The combination of negative and positive proteins in the two-block film with the insertion of an additional polyion layer to change the assembly mode: {(lysozyme/PSS)3 + PEI + (glucose oxidase/PEI)6}. The protein multicomponent films are extremely interesting as novel biologically active materials. One can arrange given protein layers according to a specific biological activity. Sequential enzymatic reactions and vectorial transfer of electrons and energy become feasible targets by the preparation of anisotropic protein layers with the precise control of the distances of active layers.

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