This chapter gives a brief description of biosensors and their existing limitations. Much emphasis has been focused on the replacement of the sensing interface with polymer nanofibers and improvements in the sensor's performance have been highlighted. The various applications into which nanofiber-based biosensors could possibly fit have also been described. It is concluded that the polymer nanofibers have a great potential in sensor applications and the research needs exploration in greater detail.

Acknowledgment. The authors would like to acknowledge the National University of Singapore (NUS), for providing us the funding and the necessary laboratory facilities. We also acknowledge all our colleagues who have helped us to a great extent in the formatting and editing of this chapter.

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