Aptamer GoldNanoparticle Conjugates for the Detection of Proteins

A highly specific sensing system for the detection of platelet-derived growth factor (PDGF) has been developed using PDGF-specific, aptamer-conjugated gold-nanoparticles.160 The principle of the detection using the conjugate is illustrated in Figure 16.8. The binding of soluble PDGF protein to Gd-NP-aptamer conjugates results in a change in the color of the solution due to the formation of aggregates between the Gd-NP conjugates. In the absence of PDGF, the conjugates are dispersed separately in solution (red), but in the presence of PDGF the Gd-NP aggregate closely to an inter-particle distances less than the average diameter of each nanoparticle, causing a dipole-dipole coupling between particles as well as increased scattering, resulting in a measurable color change (purple). - Using this detection system, Gd-NP-aptamer conjugate could detect nanomolar concentrations of PDGF in solution. Because Gd-NPs are biocompatible, easy to develop, and easy to functionalize with aptamers, it is expected that bioconjugates utilizing aptamers and Gd-NPs as target molecular probes may result in novel cancer diagnostic modalities in the future.

FIGURE 16.8 A schematic representation of the aggregation of gold-nanoparticle-aptamer conjugates in the presence of target proteins. Aggregation causes color change from red to purple.

FIGURE 16.8 A schematic representation of the aggregation of gold-nanoparticle-aptamer conjugates in the presence of target proteins. Aggregation causes color change from red to purple.

Gd-NP-Aptamer conjugates in dispersion Gd-NP-Aptamer conjugates after aggregation

Gd-NP-Aptamer conjugates in dispersion Gd-NP-Aptamer conjugates after aggregation

Diabetes Sustenance

Diabetes Sustenance

Get All The Support And Guidance You Need To Be A Success At Dealing With Diabetes The Healthy Way. This Book Is One Of The Most Valuable Resources In The World When It Comes To Learning How Nutritional Supplements Can Control Sugar Levels.

Get My Free Ebook


Post a comment