Ex Vivo

Of course, detection of bacteria both inside and outside the body is important. In a recent study, a new biological application of quantitative Raman spectroscopy was proposed to detect low levels of bacteria and inflammation (Bergholt and Hassig 2009). Native human plasma C-reactive protein (CRP) was used as a clinical bio-marker of bacterial infection and tissue damage. This protein circulates in the blood and the concentration rises as inflammation occurs. For the first time, the Raman spectrum of CRP in a buffered aqueous solution was acquired using 785 nm excitation. The concentration of CRP was measured in blood plasma, using near-infrared (NIR) Raman spectroscopy. Spectra were acquired with an in situ Inspector Raman spectrometer using 785 nm excitation. Raman spectra were collected from blood plasma drawn from 40 individuals. Quantitative predictions of CRP were made by means of partial least squares (PLS) analysis and a variable selection method Interval PLS (IPLS). The similarity of the features in the PLS regression vector to that of CRP's Raman spectrum illustrated that the prediction was sensitive to the molecular information determined by the Raman scattered light (Bergholt and Hassig 2009). The IPLS algorithm was applied to optimize the calibration model to near clinical accuracy. This study provided critical information toward the feasibility of using Raman spectroscopy for quantitative measurements of CRP in blood plasma, and, thus, to determine infection and inflammation (Fig. 3) (Bergholt and Hassig 2009).

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Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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