Protein Assays

An intermediate step between the implantation of a biomaterial and the adhesion of cells is the adsorption of proteins. After implantation, proteins rapidly adsorb to the implant surface. The type of proteins, as well as the conformation of the adsorbed proteins, is determined by the surface energy and surface roughness of the material. To promote neural cell activity and tissue regeneration, the adsorption of laminin and collagenous proteins is desirable. A NGC should be engineered to enhance and optimize protein adsorption. To evaluate a NGC candidate material, protein adsorption from a solution of laminin can be evaluated in a number of ways. A solution of a known protein concentration may be placed on a material surface and then re-analyzed for protein concentration after some of the protein has adsorbed to the material surface and, thus, is depleted from the solution. Alternatively, proteins adsorbed to a material surface can be removed by soaking in a 1% sodium dodecyl sulfate (SDS) solution, collected, and measured using a MicroBCA assay (Pierce, Rockford, Illinois) (Woo et al. 2003). Western blot analysis can be used to evaluate levels of specific proteins of interest. The quantity of proteins adsorbed to a material surface provides some insight regarding the suitability of a material for tissue growth.

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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